When observing the prepared bacterial smears at 1000x magnification, should the iris diaphragm be all the way open or stopped…

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When observing the prepared bacterial smears at 1000x magnification, should the iris diaphragm be all the way open or stopped…

When observing the prepared bacterial smears at 1000x magnification, should the iris diaphragm be all the way open or stopped down for minimal light? Why

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